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Required for mating-type switching (By similarity). 2011-01-12 The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double-strand break repair pathway in mammalian cells. Interaction of Ku with DNA is central for the functions of Ku. 2001-03-01 Ku70‐knockout mice, in contrast, appear to be tumor prone and develop thymic lymphomas with high incidence (30, 36). Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background . 2020-10-27 Ku80 is a protein that, in humans, is encoded by the XRCC5 gene.

Ku70 and ku80 function

Second, divergent genetic backgrounds or environments inﬂuence the phenotype. To distinguish between these possi-bilities, the Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks. 2013-07-01 There was no correlation between the transcript levels of Ku80 and LUC luminescence derived from T‐DNA stable transformation in KD‐OsKu80 rice calli (Figs 1b, 2d,e). It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994).

Ku70 and Ku80 (also called Ku86) are encoded by the XRCC6 and XRCC5 genes, respectively, in humans, and have a strong afﬁ nity for free ends of DNA ( 21 ). DNA-PKcs is a 469-kDa protein composed of several distinct functional domains Purification of the Ku70/Ku80 dimer. Ku70 and Ku80 were purified from the HeLa nuclear extract (CilBiotech, Mons, Belgium) by using a combination of heparin, double‐stranded DNA cellulose, phenyl sepharose high resolution and monoQ chromatographies.

DNA DAMAGE - DiVA Portal

Previous reports suggest that ku80J/J mutant mice 21 Feb 2003 Arabidopsis Ku70 and Ku80 proteins form a heterodimer with DNA binding The essential role of the Ku70/Ku80 heterodimer in telomere 26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end 24 Jul 2015 Ku80 and ku70 form the heterodimer complex Ku, required for proper Organism, UniProt Accession, Gene, Functional Information. So basically edge server plays a role to make the computational decisions ahead of time, sending only filtered data to the cloud, saving both time and money.

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1 Oct 2019 In the present study, to evaluate the role of Ku80 in thyroid carcinoma (TC), For example, the expression of Ku70/Ku80 has been shown to be 26 Mar 2002 These results suggest that the evolutionarily conserved Ku70–Ku80 heterodimer functions in DSB repair by the NHEJ pathway in A. thaliana. The information may be useful in examining the function of the Ku complex, as well Pull-down analysis with cytosolic proteins identified Ku70 and Ku80 as the The central domain of Ku70 and Ku80 is a DNA-binding beta-barrel domain. Ku makes only a few contacts with the sugar-phosphate backbone, and none with the 1 Oct 2018 Key Words: Ku70SHP-1SIRT1DNA damage repairNon-homologous end joining or DNA-targeting heterodimer (KU70 and KU80) are more sensitive to DNA To investigate the role of KU70 in response to DNA damage, we 6 Oct 2020 Knockdown of Ku70 or Ku80 in naïve mice elicited mitochondrial collapse or ER stress, leading to bronchial epithelial cell apoptosis and Together, Ku70 and Ku80 promote LPS-induced NFκB activation and break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions . 29 Aug 2018 For instance, cytoplasmic Ku70 independent of Ku80 suppresses the dimerization of Ku70 and Ku80 and to the nuclear transport function, Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase suggest that the binding of the Ku80 and Ku70 proteins to DNA plays an important role in the joining of both signal and coding ends. On the other hand, the scid previous reports that show Ku70 and Ku80 have functions independent of the Ku heterodimer [17]. Results. Previous reports suggest that ku80J/J mutant mice 21 Feb 2003 Arabidopsis Ku70 and Ku80 proteins form a heterodimer with DNA binding The essential role of the Ku70/Ku80 heterodimer in telomere 26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end 24 Jul 2015 Ku80 and ku70 form the heterodimer complex Ku, required for proper Organism, UniProt Accession, Gene, Functional Information.

It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994).
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Second, divergent genetic backgrounds or environments inﬂuence the phenotype. To distinguish between these possi-bilities, the Ku70 and Ku80 mutations were crossed together to generate Ku70, Ku80, and double Ku70 forms a heterodimer with Ku80, called Ku that is well known for repairing DNA double-strand breaks through non-homologous end joining. As a result, deletion of either causes a very similar The Ku70–Ku80 heterodimer of other species binds to the termini of double‐stranded DNA as the initial step in DSB repair. To determine whether the AtKu70–AtKu80 heterodimer also binds to DNA fragments, we performed EMSA analysis with the recombinant proteins produced in E. coli . Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96]. It is a heterodimeric protein made up of Ku70 and Ku80 [97] .

Ku70 and Ku80 form the “Ku” DNA end-binding complex which functions as the DSB recognition com-ponent of C-NHEJ, whereas the XRCC4/ Lig4 complex is specific for C-NHEJ ligation. (2002) Korabiowska et al. Modern Pathology. Ku70 and Ku80 heterodimers function as regulatory subunits of the DNA-dependent protein kinase and play a very important role in the repairing of DNA double-strand breaks. Although Ku70 is proposed as a candidate for a … Structural Basis of Importin-α-Mediated Nuclear Transport for Ku70 and Ku80 Agnes A. S. Takeda1, Andrea C. de Barros1, Chiung-Wen Chang2, Boštjan Kobe2 and Marcos R. M. Fontes1⁎ 1Departamento de Física e Biofísica, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP 18618-970, Brazil 2School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience 2003-03-01 of the Ku70/80 heterodimer in vitro. We further deﬁned the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and DNA-PKCS interaction.
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Next, the broken DNA is ligated through a complex consisting of DNA ligase IV (Lig4), XRCC4, and Cernunnos/XLF (1). Ku70‐knockout mice, in contrast, appear to be tumor prone and develop thymic lymphomas with high incidence (30, 36). Finally, in agreement with a Ku70‐specific role in life‐span regulation, it was recently shown that knockdown of Ku70, but not Ku80, significantly extended life span in the p53‐null background . XRCC4, is a protein of unknown function.

By screening a panel of uncharacterized lncRNAs to identify those whose transcription is induced by double-strand breaks (DSBs), w …. In eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end binding factor in the “classical” non-homologous end joining (C-NHEJ) pathway, the main DNA double-strand break (DSB) repair pathway in mammals. Ku, the heterodimer of Ku70 and Ku80, plays an essential role in the DNA double-strand break (DSB) repair pathway, i.e., non-homologous end-joining (NHEJ). Two isoforms of Ku80 encoded by the same genes, namely, Ku80 and KARP-1 are expressed and function in primate cells, but not in rodent cells. Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation. The Ku70 and Ku80 proteins consist of three structural domains.
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DNA repair pathways and the effect of radiotherapy in - DiVA

The atomic structure of a truncated Ku70–Ku80 was determined; however, the subunit‐specific carboxy‐terminal domain of Ku80—essential for binding to DNA‐PKcs—was determined only in isolation, and the C‐terminal domain of Ku70 Ku70 and Ku80 in A. thaliana. On the basis of their sequence similarity to the human Ku proteins, we isolated cDNAs encoding Ku70 and Ku80 homologues in this plant species (AtKu70 and AtKu80), and demonstrated that they form a functional heterodimer that both binds to double-stranded DNA and possesses DNA helicase activity. In Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal . These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other. Second, divergent genetic backgrounds or environments inﬂuence the phenotype.

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Genome-wide comprehensive analysis of human helicases

In this thesis, we have studied factors that affect tumour cell resistance to ionising radiation.

DNA repair pathways and the effect of radiotherapy in breast cancer

On the other hand, the scid previous reports that show Ku70 and Ku80 have functions independent of the Ku heterodimer [17]. Results. Previous reports suggest that ku80J/J mutant mice 21 Feb 2003 Arabidopsis Ku70 and Ku80 proteins form a heterodimer with DNA binding The essential role of the Ku70/Ku80 heterodimer in telomere 26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end 24 Jul 2015 Ku80 and ku70 form the heterodimer complex Ku, required for proper Organism, UniProt Accession, Gene, Functional Information. So basically edge server plays a role to make the computational decisions ahead of time, sending only filtered data to the cloud, saving both time and money. 24 Jul 2018 These three pins are the Switch, Output A and Output B respectively.

Function. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. The atomic structure of a truncated Ku70–Ku80 was determined; however, the subunit‐specific carboxy‐terminal domain of Ku80—essential for binding to DNA‐PKcs—was determined only in isolation, and the C‐terminal domain of Ku70 Ku70 and Ku80 in A. thaliana. On the basis of their sequence similarity to the human Ku proteins, we isolated cDNAs encoding Ku70 and Ku80 homologues in this plant species (AtKu70 and AtKu80), and demonstrated that they form a functional heterodimer that both binds to double-stranded DNA and possesses DNA helicase activity. In Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal .